Comparative studies on substrate specificity of succinic semialdehyde reductase from Gluconobacter oxydans and glyoxylate reductase from Acetobacter aceti.
Biosci Biotechnol Biochem
; 88(9): 1069-1072, 2024 Aug 26.
Article
em En
| MEDLINE
| ID: mdl-38871868
ABSTRACT
Gluconobacter oxydans succinic semialdehyde reductase (GoxSSAR) and Acetobacter aceti glyoxylate reductase (AacGR) represent a novel class in the ß-hydroxyacid dehydrogenases superfamily. Kinetic analyses revealed GoxSSAR's activity with both glyoxylate and succinic semialdehyde, while AacGR is glyoxylate specific. GoxSSAR K167A lost activity with succinic semialdehyde but retained some with glyoxylate, whereas AacGR K175A lost activity. These findings elucidate differences between these homologous enzymes.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Acetobacter
/
Gluconobacter oxydans
/
Oxirredutases do Álcool
/
Glioxilatos
Idioma:
En
Revista:
Biosci Biotechnol Biochem
Assunto da revista:
BIOQUIMICA
/
BIOTECNOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Japão