Ti4+ functionalized zirconium metal-organic frameworks with polymer brushes for specific identification of phosphopeptides in human serum and skimmed milk.
Anal Bioanal Chem
; 416(20): 4491-4501, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-38877148
ABSTRACT
In the present study, click chemistry and Schiff base reactions were simultaneously applied to prepare polymer brush (PEG)-functionalized MOF materials (UiO-66-NH2) and immobilized with Ti4+ (MOF-Brush-THBA-Ti4+) for phosphopeptide analysis. The material has a detection limit of 0.5 fmol, a selectivity of 20001, and a loading capacity of 133 mg/g for phosphopeptides. It also demonstrated great repeatability (10 cycles) and recovery rate (96.7 ± 1.4%). During the analysis of bio-samples, 4 specific phosphopeptides were identified in endogenous breast cancer serum, while 11 phosphopeptides were identified in skimmed milk. Moreover, 47 phosphopeptides correlated with 29 phosphorylated proteins were selectively identified from normal control serum, and 66 phosphopeptides correlated with 26 phosphorylated proteins were identified from breast cancer serum. Further analysis of gene ontology (GO) revealed that the detected phosphorylated proteins associated with breast cancer included positive regulation of receptor-mediated endocytosis, proteolysis, extracellular exosome, heparin binding, and chaperone binding. These findings suggest that these associated pathways might contribute to the etiology of breast cancer. Overall, this application exhibits enormous potential in the identification of phosphorylated peptides within bio-samples.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfopeptídeos
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Titânio
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Zircônio
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Leite
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Estruturas Metalorgânicas
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Anal Bioanal Chem
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Anal. bioanal. chem
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Analytical and bioanalytical chemistry
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China