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Synthesis of Biosynthetic Intermediates of Vibrioferrin and Enzyme Reactions Using Them as Substrates.
Mitome, Hidemichi; Tanabe, Tomotaka; Funahashi, Tatsuya; Akira, Kazuki.
Afiliação
  • Mitome H; Laboratory of Pharmaceutical Analytical Chemistry, College of Pharmaceutical Sciences, Matsuyama University.
  • Tanabe T; Laboratory of Hygienic Chemistry, College of Pharmaceutical Sciences, Matsuyama University.
  • Funahashi T; Laboratory of Hygienic Chemistry, College of Pharmaceutical Sciences, Matsuyama University.
  • Akira K; Laboratory of Pharmaceutical Analytical Chemistry, College of Pharmaceutical Sciences, Matsuyama University.
Chem Pharm Bull (Tokyo) ; 72(6): 559-565, 2024.
Article em En | MEDLINE | ID: mdl-38880627
ABSTRACT
Biosynthetic intermediates of siderophore vibrioferrin (VF), O-citryl-L-serine, 2-aminoethyl citrate, and alanine-2-amidoethyl citrate were respectively synthesized as a mixture of stereoisomers. These compounds were used as substrates for enzyme reactions using recombinant PvsA, PvsB, and PvsE proteins as corresponding enzyme equivalents. The results of our study show that each enzyme reacts with a respective substrate and produces VF along the proposed biosynthetic pathway. Furthermore, the results of this study will contribute to the understanding of VF biosynthetic enzymes and may help in the development of antimicrobial drugs by inhibiting siderophore biosynthetic enzymes.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sideróforos Idioma: En Revista: Chem Pharm Bull (Tokyo) Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Sideróforos Idioma: En Revista: Chem Pharm Bull (Tokyo) Ano de publicação: 2024 Tipo de documento: Article