Identification and Characterization of Antigen-Specific T-Cells in Viral Infections.
Methods Mol Biol
; 2813: 295-308, 2024.
Article
em En
| MEDLINE
| ID: mdl-38888785
ABSTRACT
Identification and characterization of CD8+ T-cells is important to determine their role in protecting and clearing viral infections. Here we provide details of the peptide-MHC (pMHC) tetramers-based approach to identify antigen-specific T-cells in human and murine samples. This method provides ex vivo quantification and functional characterization of T-cells reactive to specific viral antigens derived from CMV and rotavirus in human blood and in murine intestinal lamina propria samples, respectively.
Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rotavirus
/
Linfócitos T CD8-Positivos
/
Antígenos Virais
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Dinamarca