Robust CRISPR/Mb2Cas12a genome editing tools in cotton plants.
Imeta
; 3(3): e209, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-38898989
ABSTRACT
The efficiency and accuracy of the CRISPR/Mb2Cas12a system were demonstrated in cotton, achieving an efficiency of over 90% at target sites. Notably, Mb2Cas12a exhibited significant tolerance under different temperatures ranging from 22°C to 32°C. Additionally, the Mb2Cas12a system revealed effective editing at more relaxed VTTV PAM sites in the cotton genome, which expanded the genome editing range by approximately 2.6-fold than the wide-type LbCas12a. Finally, a multiplex genome editing system was also developed based on Mb2Cas12a, enabling simultaneous editing of eight target sites using a single crRNA cassette.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
Imeta
Ano de publicação:
2024
Tipo de documento:
Article