Dynamic Foxp3-chromatin interaction controls tunable Treg cell function.
J Exp Med
; 221(9)2024 09 02.
Article
em En
| MEDLINE
| ID: mdl-38935023
ABSTRACT
Nuclear factor Foxp3 determines regulatory T (Treg) cell fate and function via mechanisms that remain unclear. Here, we investigate the nature of Foxp3-mediated gene regulation in suppressing autoimmunity and antitumor immune response. Contrasting with previous models, we find that Foxp3-chromatin binding is regulated by Treg activation states, tumor microenvironment, and antigen and cytokine stimulations. Proteomics studies uncover dynamic proteins within Foxp3 proximity upon TCR or IL-2 receptor signaling in vitro, reflecting intricate interactions among Foxp3, signal transducers, and chromatin. Pharmacological inhibition and genetic knockdown experiments indicate that NFAT and AP-1 protein Batf are required for enhanced Foxp3-chromatin binding in activated Treg cells and tumor-infiltrating Treg cells to modulate target gene expression. Furthermore, mutations at the Foxp3 DNA-binding domain destabilize the Foxp3-chromatin association. These representative settings delineate context-dependent Foxp3-chromatin interaction, suggesting that Foxp3 associates with chromatin by hijacking DNA-binding proteins resulting from Treg activation or differentiation, which is stabilized by direct Foxp3-DNA binding, to dynamically regulate Treg cell function according to immunological contexts.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cromatina
/
Linfócitos T Reguladores
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Fatores de Transcrição Forkhead
Limite:
Animals
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Humans
Idioma:
En
Revista:
J Exp Med
/
J. exp. med
/
Journal of experimental medicine
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos