Protocol for the collection and analysis of the different immune cell subsets in the murine intestinal lamina propria.
STAR Protoc
; 5(3): 103154, 2024 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-38935510
ABSTRACT
The intestinal lamina propria (LP) is a leukocyte-rich cornerstone of the immune system owing to its vital role in immune surveillance and barrier defense against external pathogens. Here, we present a protocol for isolating and analyzing immune cell subsets from the mouse intestinal LP for further downstream applications. Starting from tissue collection and cleaning, epithelium removal, and enzymatic digestion to collection of single cells, we explain each step in detail to maximize the yield of immune cells from the intestinal LP.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mucosa Intestinal
Limite:
Animals
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article