Visualization of cysteine in AD mouse with a high-quantum yield NIR fluorescent probe.
Talanta
; 278: 126482, 2024 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-38950502
ABSTRACT
Alzheimer's disease (AD) has gradually received enthusiastic attention with the aging process, and studying its biological relevance is expected. Excitingly, fluorescence probes were considered to be powerful tools for exploring biological correlations. Therefore, a highly selective near-infrared (NIR) fluorescent probe (DCM-Cl-Acr) for imaging cysteine (Cys) in AD was designed and synthesized. Through structural optimization, the probe exhibited high fluorescence quantum yield and low detection limit (20 nM) towards Cys. Meanwhile, based on the high selectivity and high sensitivity response exhibited by the probe to Cys, it was successfully applied to visualize endogenous and exogenous Cys in living cells and zebrafish, and showed good discrimination from homocysteine (Hcy) and glutathione (GSH). Further, the correlation between AD and Cys concentration was clarified by imaging studies in hippocampus tissue of AD mouse, and the abnormal accumulation of Cys in the hippocampus of AD brain was demonstrated.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
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Cisteína
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Doença de Alzheimer
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Corantes Fluorescentes
Limite:
Animals
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Humans
Idioma:
En
Revista:
Talanta
Ano de publicação:
2024
Tipo de documento:
Article