Evaluation of the FPMC respiratory panel for detection of respiratory tract pathogens in nasopharyngeal swab and sputum specimens.
Virol J
; 21(1): 156, 2024 Jul 11.
Article
em En
| MEDLINE
| ID: mdl-38992721
ABSTRACT
OBJECTIVES:
The performance of the new Respiratory Pathogen panel (fluorescent probe melting curve, FPMC) for the qualitative detection of 12 organisms (chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, influenza A virus, influenza B virus, parainfluenza virus, rhinovirus, etc.) was assessed.METHODS:
Prospectively collected nasopharyngeal swab (NPS) and sputum specimens (n = 635) were detected by using the FPMC panel, with the Sanger sequencing method as the comparative method.RESULTS:
The overall percent concordance between the FPMC analysis method and the Sanger sequencing method was 100% and 99.66% for NPS and sputum specimens, respectively. The FPMC testified an overall positive percent concordance of 100% for both NPS and sputum specimens. The FPMC analysis method also testified an overall negative percent concordance of 100% and 99.38% for NPS and sputum specimens, respectively.CONCLUSIONS:
The FPMC analysis method is a stable and accurate assay for rapid, comprehensive detecting for respiratory pathogens.Palavras-chave
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Infecções Respiratórias
/
Escarro
/
Nasofaringe
/
Técnicas de Diagnóstico Molecular
Limite:
Adolescent
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Adult
/
Aged
/
Aged80
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Child
/
Child, preschool
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Female
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Humans
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Infant
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Male
Idioma:
En
Revista:
Virol J
Assunto da revista:
VIROLOGIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China