Recovery from cold-induced mitochondrial fission in endothelial cells requires reconditioning temperatures of ≥ 25â¦C.
Front Transplant
; 1: 1044551, 2022.
Article
em En
| MEDLINE
| ID: mdl-38994396
ABSTRACT
Mitochondrial integrity and function constitute a prerequisite for cellular function and repair processes. We have previously shown that mitochondria of different cell types exhibit pronounced fragmentation under hypothermic conditions. This fission, accompanied by a decline of cellular ATP content, showed reversibility at 37â¦C. However, it is unclear whether other temperatures as currently discussed for reconditioning of organs allow this reconstitution of mitochondria. Therefore, we here study in a model of cultured porcine aortic endothelial cells how different rewarming temperatures affect mitochondrial re-fusion and function. After 48 h cold incubation of endothelial cells in Krebs-Henseleit buffer with glucose (5 mM) and deferoxamine (1 mM) at 4â¦C pronounced mitochondrial fission was observed. Following 2 h rewarming in cell culture medium, marked fission was still present after rewarming at 10⦠or 15â¦C. At 21â¦C some re-fusion was visible, which became more marked at 25â¦C. Networks of tubular mitochondria similar to control cells only re-appeared at 37â¦C. ATP content decreased at 4â¦C from 3.6 ± 0.4 to 1.6 ± 0.4 nmol/106 cells and decreased even further when rewarming cells to 10⦠and 15â¦C. Values after rewarming at 21â¦C were similar to the values before rewarming while ATP gradually increased at higher rewarming temperatures. Metabolic activity dropped to 5 ± 11% of control values during 4â¦C incubation and recovered with increasing temperatures to 36 ± 10% at 25â¦C and 78 ± 17% at 37â¦C. Integrity of monolayers, largely disturbed at 4â¦C (large gaps between endothelial cells; cell injury ≤ 1%), showed partial recovery from 15â¦C upwards, complete recovery at 37â¦C. Endothelial repair processes (scratch assay) at 25â¦C were clearly inferior to those at 37â¦C. These data suggest that reconditioning temperatures below 21â¦C are not optimal with regard to reconstitution of mitochondrial integrity and function. For this goal, temperatures of at least 25â¦C appear required, with 30â¦C being superior and 37â¦C yielding the best results.
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MEDLINE
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En
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Front Transplant
Ano de publicação:
2022
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Article
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Alemanha