Histone variant H2BE enhances chromatin accessibility in neurons to promote synaptic gene expression and long-term memory.
Mol Cell
; 84(15): 2822-2837.e11, 2024 Aug 08.
Article
em En
| MEDLINE
| ID: mdl-39025074
ABSTRACT
Histone proteins affect gene expression through multiple mechanisms, including through exchange with histone variants. Recent findings link histone variants to neurological disorders, yet few are well studied in the brain. Most notably, widely expressed variants of H2B remain elusive. We applied recently developed antibodies, biochemical assays, and sequencing approaches to reveal broad expression of the H2B variant H2BE and defined its role in regulating chromatin structure, neuronal transcription, and mouse behavior. We find that H2BE is enriched at promoters, and a single unique amino acid allows it to dramatically enhance chromatin accessibility. Further, we show that H2BE is critical for synaptic gene expression and long-term memory. Together, these data reveal a mechanism linking histone variants to chromatin accessibility, transcriptional regulation, neuronal function, and memory. This work further identifies a widely expressed H2B variant and uncovers a single histone amino acid with profound effects on genomic structure.
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Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sinapses
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Cromatina
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Histonas
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Memória de Longo Prazo
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Neurônios
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Mol Cell
/
Mol. cell
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Molecular cell
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2024
Tipo de documento:
Article