Light-emitting probes for in situ sensing of plant information.

ABSTRACT

Monitoring plant physiological information for gaining a comprehensive understanding of plant growth and stress responses contributes to safeguarding plant health. Light-emitting probes - in terms of small-molecule, nanomaterials-based, and genetically protein-based probes - can be introduced into plants through foliar and root treatment or genetic transformation. These probes offer exciting opportunities for sensitive and in situ monitoring of dynamic plant chemical information - for example, reactive oxygen species (ROS), calcium ions, phytohormones - with spatiotemporal resolution. In this review we explore the sensing mechanisms of these light-emitting probes and their applications in monitoring various chemical information in plants in situ. These probes can be used as part of a sentinel plant approach to provide stress warning in the field or to explore plant signaling pathways.