Salmonella Typhimurium exploits host polyamines for assembly of the type 3 secretion machinery.
PLoS Biol
; 22(8): e3002731, 2024 Aug.
Article
em En
| MEDLINE
| ID: mdl-39102375
ABSTRACT
Bacterial pathogens utilize the factors of their hosts to infect them, but which factors they exploit remain poorly defined. Here, we show that a pathogenic Salmonella enterica serovar Typhimurium (STm) exploits host polyamines for the functional expression of virulence factors. An STm mutant strain lacking principal genes required for polyamine synthesis and transport exhibited impaired infectivity in mice. A polyamine uptake-impaired strain of STm was unable to inject effectors of the type 3 secretion system into host cells due to a failure of needle assembly. STm infection stimulated host polyamine production by increasing arginase expression. The decline in polyamine levels caused by difluoromethylornithine, which inhibits host polyamine production, attenuated STm colonization, whereas polyamine supplementation augmented STm pathogenesis. Our work reveals that host polyamines are a key factor promoting STm infection, and therefore a promising therapeutic target for bacterial infection.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Poliaminas
/
Salmonella typhimurium
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Fatores de Virulência
/
Sistemas de Secreção Tipo III
Limite:
Animals
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Female
/
Humans
Idioma:
En
Revista:
PLoS Biol
Assunto da revista:
BIOLOGIA
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Japão