A microRNA396b-growth regulating factor module controls castor seed size by mediating auxin synthesis.
Plant Physiol
; 196(2): 916-930, 2024 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-39140314
ABSTRACT
Castor (Ricinus communis L.) is an importance crop cultivated for its oil and economic value. Seed size is a crucial factor that determines crop yield. Gaining insight into the molecular regulatory processes of seed development is essential for the genetic enhancement and molecular breeding of castor. Here, we successfully fine-mapped a major QTL related to seed size, qSS3, to a 180â
kb interval on chromosome 03 using F2 populations (DL01×WH11). A 17.6-kb structural variation (SV) was detected through genomic comparison between DL01 and WH11. Analysis of haplotypes showed that the existence of the complete 17.6â
kb structural variant may lead to the small seed characteristic in castor. In addition, we found that qSS3 contains the microRNA396b (miR396b) sequence, which is situated within the 17.6â
kb SV. The results of our experiment offer additional evidence that miR396-Growth Regulating Factor 4 (GRF4) controls seed size by impacting the growth and multiplication of seed coat and endosperm cells. Furthermore, we found that RcGRF4 activates the expression of YUCCA6 (YUC6), facilitating the production of IAA in seeds and thereby impacting the growth of castor seeds. Our research has discovered a crucial functional module that controls seed size, offering a fresh understanding of the mechanism underlying seed size regulation in castor.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sementes
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Regulação da Expressão Gênica de Plantas
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MicroRNAs
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Ácidos Indolacéticos
Idioma:
En
Revista:
Plant Physiol
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
China