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Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research.
Casabianca, Anthony; Bellina, Mélanie; Dudgeon, Crissy; Nezhad, Muhammad Sadeqi; Harris, Chris; Fiore, Stephany; Bernet, Agnes; Narrow, Wade; Prela, Orjola; Wang, Lan; Astsaturov, Igor; Mehlen, Patrick; Carpizo, Darren R.
Afiliação
  • Casabianca A; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.
  • Bellina M; Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netri
  • Dudgeon C; Rutgers Cancer Institute of New Jersey, New Brunswick, NJ 08901, USA.
  • Nezhad MS; University of Rochester Clinical and Translational Science Institute, Rochester, NY 14620, USA.
  • Harris C; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA.
  • Fiore S; Netris Pharma, 69008 Lyon. France.
  • Bernet A; Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netri
  • Narrow W; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA.
  • Prela O; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.
  • Wang L; Department of Pathology and Laboratory Medicine, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA.
  • Astsaturov I; Department of Medical Oncology, Fox Chase Cancer Center, Philadephia, PA 19111, USA.
  • Mehlen P; Apoptosis, Cancer and Development Laboratory - Equipe labellisée 'La Ligue', LabEx DEVweCAN, Institut Convergence PLAsCAN, Centre de Recherche en Cancérologie de Lyon (CRCL), INSERM U1052-CNRS UMR5286, Université de Lyon, Université Claude Bernard Lyon1, Centre Léon Bérard, 69008 Lyon, France; Netri
  • Carpizo DR; Department of Surgery, Division of Surgical Oncology, University of Rochester School of Medicine and Dentistry, Rochester, NY 14620, USA; Wilmot Cancer Center, University of Rochester, Rochester, NY 14620, USA. Electronic address: darren_carpizo@urmc.rochester.edu.
STAR Protoc ; 5(3): 103256, 2024 Aug 13.
Article em En | MEDLINE | ID: mdl-39141519
ABSTRACT
Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.1.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos