Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research.
STAR Protoc
; 5(3): 103256, 2024 Aug 13.
Article
em En
| MEDLINE
| ID: mdl-39141519
ABSTRACT
Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.1.
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Base de dados:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Estados Unidos