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Cell-substrate distance fluctuations of confluent cells enable fast and coherent collective migration.
Jipp, Marcel; Wagner, Bente D; Egbringhoff, Lisa; Teichmann, Andreas; Rübeling, Angela; Nieschwitz, Paul; Honigmann, Alf; Chizhik, Alexey; Oswald, Tabea A; Janshoff, Andreas.
Afiliação
  • Jipp M; University of Göttingen, Institute of Physical Chemistry, Tammannstrasse 6, 37077 Göttingen, Germany.
  • Wagner BD; University of Göttingen, Institute of Physical Chemistry, Tammannstrasse 6, 37077 Göttingen, Germany.
  • Egbringhoff L; University of Göttingen, Institute of Physical Chemistry, Tammannstrasse 6, 37077 Göttingen, Germany.
  • Teichmann A; University of Göttingen, Institute of Physical Chemistry, Tammannstrasse 6, 37077 Göttingen, Germany.
  • Rübeling A; University of Göttingen, Institute of Organic and Biomolecular Chemistry, Tammannstrasse 2, 37077 Göttingen, Germany.
  • Nieschwitz P; University of Göttingen, Institute of Physical Chemistry, Tammannstrasse 6, 37077 Göttingen, Germany.
  • Honigmann A; Biotechnology Center, Technische Universität Dresden, Pfotenhauerstrasse 108, 01307 Dresden, Germany.
  • Chizhik A; University of Göttingen, Third Institute of Physics, Friedrich-Hund-Platz 1, 37077 Göttingen, Germany.
  • Oswald TA; University of Göttingen, Institute of Organic and Biomolecular Chemistry, Tammannstrasse 2, 37077 Göttingen, Germany. Electronic address: toswald@gwdg.de.
  • Janshoff A; University of Göttingen, Institute of Physical Chemistry, Tammannstrasse 6, 37077 Göttingen, Germany. Electronic address: ajansho@gwdg.de.
Cell Rep ; 43(8): 114553, 2024 Aug 27.
Article em En | MEDLINE | ID: mdl-39150846
ABSTRACT
Collective cell migration is an emergent phenomenon, with long-range cell-cell communication influenced by various factors, including transmission of forces, viscoelasticity of individual cells, substrate interactions, and mechanotransduction. We investigate how alterations in cell-substrate distance fluctuations, cell-substrate adhesion, and traction forces impact the average velocity and temporal-spatial correlation of confluent monolayers formed by either wild-type (WT) MDCKII cells or zonula occludens (ZO)-1/2-depleted MDCKII cells (double knockdown [dKD]) representing highly contractile cells. The data indicate that confluent dKD monolayers exhibit decreased average velocity compared to less contractile WT cells concomitant with increased substrate adhesion, reduced traction forces, a more compact shape, diminished cell-cell interactions, and reduced cell-substrate distance fluctuations. Depletion of basal actin and myosin further supports the notion that short-range cell-substrate interactions, particularly fluctuations driven by basal actomyosin, significantly influence the migration speed of the monolayer on a larger length scale.
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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Adesão Celular / Movimento Celular Limite: Animals Idioma: En Revista: Cell Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Adesão Celular / Movimento Celular Limite: Animals Idioma: En Revista: Cell Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Alemanha