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Preliminary construction of non-coding RNAs and ceRNA regulatory networks mediated by exosomes in porcine follicular fluid.
Liu, Yangguang; Xie, Fan; Zhang, Huibin; Ye, Haibo; Wen, Haoyu; Qiu, Mengyao; Ding, Yueyun; Zheng, Xianrui; Yin, Zongjun; Zhang, Xiaodong.
Afiliação
  • Liu Y; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
  • Xie F; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
  • Zhang H; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
  • Ye H; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
  • Wen H; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
  • Qiu M; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China. Electronic address: 1679854821@qq.com.
  • Ding Y; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
  • Zheng X; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China.
  • Yin Z; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China. Electronic address: yinzongjun@ahau.ed
  • Zhang X; College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui 230036, China; Anhui Province Key Laboratory of Local Livestock and Poultry Genetic Resource Conservation and Bio-Breeding, Anhui Agricultural University, Hefei 230036, China. Electronic address: xdzhang1983@ahau.e
Genomics ; 116(5): 110920, 2024 Aug 14.
Article em En | MEDLINE | ID: mdl-39151553
ABSTRACT

BACKGROUND:

Follicles are fundamental units of the ovary, regulated intricately during development. Exosomes and ovarian granulosa cells (OGCs) play pivotal roles in follicular development, yet the regulatory mechanisms governing exosomes remain elusive.

RESULTS:

High-throughput sequencing was employed to evaluate the complete transcript expression profiles of six samples (three porcine ovarian granulosa cells-exosome co-culture samples (GCE) and three porcine ovarian granulosa cells (POGCs) samples). Differential expression analysis revealed 924 lncRNAs, 35 circRNAs, 49 miRNAs, and 9823 mRNAs in the GCE group. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated enrichment of differentially expressed transcripts in pathways related to cell proliferation and apoptosis. Furthermore, a ceRNA regulatory network comprising 43 lncRNAs, 6 circRNAs, 11 miRNAs, and 126 mRNAs was constructed based on intergene co-expression correlations. Seven miRNAs associated with cell proliferation and apoptosis regulation were identified within this network, encompassing 92 subnet pairs as candidate genes for further exploration of exosome regulatory mechanisms. Additionally, preliminary verification at the cellular level demonstrated that exosomal miR-200b enhances the viability of POGCs.

CONCLUSIONS:

Transcriptome analysis unveiled a pivotal candidate ceRNA network potentially implicated in exosome-mediated regulation of granulosa cell proliferation and apoptosis, thereby influencing porcine follicular development. These findings offer insights into the molecular mechanisms of follicular fluid exosome regulation, encompassing both coding and non-coding RNA perspectives.
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Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Genomics Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Base de dados: MEDLINE Idioma: En Revista: Genomics Assunto da revista: GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China