In situ imaging of LPMO action on plant tissues.
Carbohydr Polym
; 343: 122465, 2024 Nov 01.
Article
em En
| MEDLINE
| ID: mdl-39174080
ABSTRACT
Lytic polysaccharide monooxygenases (LPMOs) are copper-dependent enzymes that oxidatively cleave recalcitrant polysaccharides such as cellulose. Several studies have reported LPMO action in synergy with other carbohydrate-active enzymes (CAZymes) for the degradation of lignocellulosic biomass but direct LPMO action at the plant tissue level remains challenging to investigate. Here, we have developed a MALDI-MS imaging workflow to detect oxidised oligosaccharides released by a cellulose-active LPMO at cellular level on maize tissues. Using this workflow, we imaged LPMO action and gained insight into the spatial variation and relative abundance of oxidised and non-oxidised oligosaccharides. We reveal a targeted action of the LPMO related to the composition and organisation of plant cell walls.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Zea mays
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Oxigenases de Função Mista
Idioma:
En
Revista:
Carbohydr Polym
Ano de publicação:
2024
Tipo de documento:
Article