Differential effects of dimethylsulfoxide on the activities of human DNA polymerases alpha and delta.
Nucleic Acids Res
; 14(4): 1719-26, 1986 Feb 25.
Article
em En
| MEDLINE
| ID: mdl-3951994
ABSTRACT
The effects of dimethylsulfoxide on the activities of purified human placental DNA polymerase alpha and DNA polymerase delta were examined. DNA polymerase alpha was inhibited by dimethylsulfoxide, whereas DNA polymerase delta was significantly activated, by as much as 6-fold. Kinetic data show that the effect of dimethylsulfoxide on DNA polymerase delta activity was due to a reduction in the apparent Km for its substrate, dTTP. This novel finding of the differential effects of dimethylsulfoxide on the activities of polymerases alpha and delta may be useful in their identification and differential assay.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Dimetil Sulfóxido
/
DNA Polimerase II
/
DNA Polimerase Dirigida por DNA
/
Replicação do DNA
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Nucleic Acids Res
Ano de publicação:
1986
Tipo de documento:
Article