Evidence for the formation of inositol 4-monophosphate in stimulated human platelets.
FEBS Lett
; 185(1): 151-6, 1985 Jun 03.
Article
em En
| MEDLINE
| ID: mdl-3996593
ABSTRACT
Human platelets were prelabeled with [3H] inositol and exposed to thrombin or vasopressin. The radioactive inositol monophosphates were separated by high-performance liquid chromatography and identified by cochromatography with unlabeled standard substances. Radioactive inositol 1-monophosphate (Ins 1-P) and inositol 4-monophosphate (Ins 4-P) were detected in unstimulated platelets and accumulated in response to thrombin or vasopressin. Ins 4-P as well as Ins 1-P increased after the formation of inositol 1,4-bisphosphate (Ins 1,4-P2) and inositol 1,4,5-trisphosphate (Ins 1,4,5-P3). Lithium augmented the accumulation of Ins 1-P and Ins 1,4-P2 in stimulated platelets, and also of Ins 4-P in platelets stimulated by vasopressin, but not by thrombin. The results indicate that Ins 1,4-P2 formed in stimulated platelets is partly degraded to Ins 4-P. The significance of Ins 4-P as a marker molecule for the study of inositol phosphate metabolism in stimulated cells is discussed.
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Base de dados:
MEDLINE
Assunto principal:
Fosfatos Açúcares
/
Plaquetas
/
Vasopressinas
/
Trombina
/
Fosfatos de Inositol
Limite:
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1985
Tipo de documento:
Article