Murine primitive neuroectodermal tumor: nucleolar isolation and RNA polymerase inhibition.
Experientia
; 35(5): 660-2, 1979 May 15.
Article
em En
| MEDLINE
| ID: mdl-446675
ABSTRACT
A nucleolar isolation procedure was developed for the murine primitive neuroectodermal tumor originally induced with methylcholanthrene. This procedure utilizes sonication for breakage of the nuclei at a magnesium concentration which disperses the extranucleolar chromatin. The nucleoli retain RNA synthetic activity which is inhibited to about 90% by actinomycin D and the chemotherapeutic drug adriamycin.
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Base de dados:
MEDLINE
Assunto principal:
RNA Polimerase I
/
RNA Polimerases Dirigidas por DNA
/
Nucléolo Celular
/
Ependimoma
Limite:
Animals
Idioma:
En
Revista:
Experientia
Ano de publicação:
1979
Tipo de documento:
Article