Applicability of aeration and delayed addition of selenite to the isolation of salmonellae.
Appl Microbiol
; 18(6): 1060-4, 1969 Dec.
Article
em En
| MEDLINE
| ID: mdl-4905697
ABSTRACT
The effect of aeration combined with the delayed addition of selenite on the lag period of several strains of salmonellae and other enterobacteria is reported. A procedure has been developed involving shaking of the sample in a basal medium for 4 hr at 37 C, adding selenite and cystine, and continuing shaking for 20 hr. Confirmation by selective plating, biochemical tests, and serology gave results comparable to the standard lactose pre-enrichment method with the saving of 24 hr and elimination of one set of media. Confirmation by fluorescent-antibody tests showed that fewer positive fluorescent stains were obtained from the aerated procedure than from the lactose pre-enrichment procedure. Precautions in the application of this procedure are discussed.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Salmonella
/
Selênio
/
Microbiologia de Alimentos
Idioma:
En
Revista:
Appl Microbiol
Ano de publicação:
1969
Tipo de documento:
Article