Colorimetric determination of caffeic acid in plant materials.
J Assoc Off Anal Chem
; 62(5): 1160-1, 1979 Sep.
Article
em En
| MEDLINE
| ID: mdl-528465
ABSTRACT
A new colorimetric method is described for determining caffeic acid content in plant materials. Caffeic acid is separated by thin layer chromatography from the alcoholic extract, and color is developed using 0.5% aqueous thiosemicarbazide solution under alkaline conditions. The absorbance is read at 475 nm. Lambert-Beer's law is obeyed in the concentration range 0.37-17.5 micrograms caffeic acid/mL. The method is reproducible and has been applied to the estimation of caffeic acid in carrot roots.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Verduras
/
Ácidos Cafeicos
/
Cinamatos
Idioma:
En
Revista:
J Assoc Off Anal Chem
Ano de publicação:
1979
Tipo de documento:
Article