[Occurrence and behavior of fibrin fibrinogen degradation products in concentrates of factor VIII, cryoprecipitate and dextran precipitate during long-term preservation]. / Vorkommen und Verhalten von Fibrinogenspaltprodukten in den Faktor-VIII-konzentraten Kryopräzipitat und Dextranpräzipitat bei Langzeitlagerung.

ABSTRACT

In deep-frozen and lyophilized cryoprecipitates and dextran precipitates it was analyzed by the TRCHII and the SCT, whether these factor VIII concentrates contain fibrinogen fission products effecting as inhibitors of the coagulation of blood and how they act during storage for 200 days. Simultaneously the parameters total protein, fibrinogen, fibrin monomers, plasminogen and factor VIII activity were always determined. In all preparations being tested fibrinogen fission products could be detected. Lyophilized cryoprecipitates showed the highest average values with 7.97 microgram/ml (TRCHII) resp. 18.31 microgram/ml (SCT). They were followed by deep-frozen cryoprecipitates with 6.93 microgram/ml (TRCHII) resp. 7.54 microgram/ml (SCT). Deep-frozen dextran precipitates free of heparin contained the fewest fission products (TRCHII 0.36 microgram/ml; SCT 3.13 microgram/ml). At the beginning of the storage only few fission products were found in all groups of preparates. A predominantly clear rise with forming a peak after about 115 to 145 days and a drop following happened with extended storage period. This course of storage of the quantities of fission products shows that the fibrinogen fission products arise during storage.