Rapid restriction mapping of DNA cloned in lambda phage vectors.
Gene
; 30(1-3): 195-200, 1984 Oct.
Article
em En
| MEDLINE
| ID: mdl-6096214
ABSTRACT
A protocol for the rapid restriction mapping of phage lambda clones has been developed. Partial digestion products are selectively labelled at the right or left cohesive lambda DNA termini by hybridisation with [32P]oligonucleotides complementary to the single-stranded cos ends. After gel electrophoresis and autoradiography, the restriction map can be directly determined from the "ladder" of partial digestion products.
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Base de dados:
MEDLINE
Assunto principal:
DNA Viral
/
Mapeamento Cromossômico
/
Bacteriófago lambda
Idioma:
En
Revista:
Gene
Ano de publicação:
1984
Tipo de documento:
Article