T lymphocyte-mediated suppression of myeloma function in vitro. IV. Generation of effector suppressor cells specific for myeloma idiotypes.
J Exp Med
; 155(4): 1216-21, 1982 Apr 01.
Article
em En
| MEDLINE
| ID: mdl-6174675
ABSTRACT
BALB/c mice immunized intravenously with syngeneic splenocytes, to which affinity-purified IgA produced by the MOPC 315 myeloma is covalently coupled, develop suppressor T cells (Ts1) that inhibit IgA secretion by MOPC 315 cells after 3-4 d of co-culture. Immunization with M315-coupled splenocytes subcutaneously, followed by administration of a soluble extract of Ts1 cells, leads to the generation of effector Ts that are also idiotype specific and inhibit myeloma function within 1 d. Moreover, effector Ts are Lyt-1-2+, whereas Ts1 are either Lyt-1+2+ or require Lyt-1+ and Lyt-2+ cells to mature into effector Ts in vitro. Such a protocol should be useful for analyzing the interactions that result in the maturation of Ts and in defining the mechanisms of action of Ts, whose effect can be measured on a homogeneous target population and that are specific for a well-characterized myeloma idiotype.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Plasmocitoma
/
Linfócitos T Reguladores
/
Idiótipos de Imunoglobulinas
/
Epitopos
Limite:
Animals
Idioma:
En
Revista:
J Exp Med
Ano de publicação:
1982
Tipo de documento:
Article