Differential expression of the actin gene family of Strongylocentrotus purpuratus.
Dev Biol
; 101(2): 295-306, 1984 Feb.
Article
em En
| MEDLINE
| ID: mdl-6198225
ABSTRACT
Molecular probes that individually recognize the 3' nontranslated regions of six actin genes were utilized in RNA gel blot hybridizations to detect RNAs complementary to each gene in embryonic and adult tissues of Strongylocentrotus purpuratus. In addition the probes were used in DNA excess filter hybridizations to estimate the relative contribution of the different actin genes. All six genes produce relatively stable mRNAs, and each displays a characteristic and distinct pattern of expression. On the basis of their expression in the egg, early embryos, or in adult coelomocytes, it is concluded that genes termed CyI, CyIIa, CyIIb, CyIIIa, and CyIIIb encode cytoskeletal actin proteins. Actin gene M gives rise to mRNAs that are found only in tissues containing muscle. Actin genes CyI, CyIIa, CyIIb, and M are expressed in both adult and embryonic tissues, giving rise to transcripts 2.1-2.2 kb in length. Expression of genes CyIIIa and CyIIIb is confined to the embryo. Gene CyIIIa provides the major embryonic actin mRNA, which is 1.8 kb in length. Three of the cytoskeletal actin genes are linked over a 30-kb distance in the S. purpuratus genome. We show that the actin genes included in this linkage group are not coordinately expressed.
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Base de dados:
MEDLINE
Assunto principal:
Ouriços-do-Mar
/
Regulação da Expressão Gênica
/
Actinas
Limite:
Animals
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
1984
Tipo de documento:
Article