Molecular cloning of the "minor" component of the kinetoplast DNA of Trypanosoma lewisi.

Ponzi, M; Del Bue, M; Ottaviano, M; Battaglia, P A

Ponzi, M; Del Bue, M; Ottaviano, M; Battaglia, P A.

Cell Biol Int Rep ; 4(12): 1109-16, 1980 Dec.

Article em En | MEDLINE | ID: mdl-6257401

RESUMO

Kinetoplast DNA was cloned in the pBR322 plasmid using the BamHI site. In situ hybridization of iodinated recombinant DNA showed hybridization grains only in the kinetoplast region. The recombinant DNA was analyzed with restriction enzymes and its base ratio was determined by analytical CsCl density gradient.

Assuntos

Clonagem Molecular; DNA Mitocondrial/análise; Trypanosoma lewisi/análise; Animais; Composição de Bases; Sequência de Bases; Centrifugação com Gradiente de Concentração; Enzimas de Restrição do DNA; Peso Molecular; Hibridização de Ácido Nucleico

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Base de dados: MEDLINE Assunto principal: Trypanosoma lewisi / DNA Mitocondrial / Clonagem Molecular Limite: Animals Idioma: En Revista: Cell Biol Int Rep Ano de publicação: 1980 Tipo de documento: Article

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