Coxsackievirus B-3 myocarditis in Balb/c mice. Evidence for autoimmunity to myocyte antigens.
Am J Pathol
; 116(1): 21-9, 1984 Jul.
Article
em En
| MEDLINE
| ID: mdl-6331168
ABSTRACT
Male Balb/c mice inoculated with a heart-adapted variant of Coxsackievirus, group B, type 3 (Nancy) (CVB3M), develop extensive myocarditis and cytolytic activity to primary cultures of uninfected and infected myocytes. To elucidate the mechanisms of myocyte injury in myocarditis, two distinct cytolytic T-lymphocyte (CTL) populations were isolated by immunoadsorption of lymph node cells to glutaraldehyde-fixed uninfected and infected myocyte monolayers. One population preferentially adsorbed to and lysed uninfected myocytes (autoreactive CTLs), while the other adsorbed to and lysed CVB3M-infected myocytes (virus-specific CTL). Neither CTL population adsorbed to monolayers of HeLa, L929, or umbilical cord endothelial cells, or to myocytes infected with a related but nonmyocarditic Coxsackievirus B-3 variant ( CVB3o ). While both autoreactive and virus-specific CTLs induced myocarditis in vivo, the lesions caused by autoreactive CTLs were more extensive and necrotizing than those of virus-specific cells. These results support the hypothesis that CVB3 -induced myocarditis results, in part, from autoimmunity to myocyte antigens.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Autoanticorpos
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Autoantígenos
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Linfócitos T Citotóxicos
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Infecções por Coxsackievirus
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Miocardite
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Miocárdio
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Antígenos
Limite:
Animals
Idioma:
En
Revista:
Am J Pathol
Ano de publicação:
1984
Tipo de documento:
Article