Identification of a heparin activated amidolytic enzyme in carp (Cyprinus carpio) plasma.
Thromb Res
; 29(3): 289-97, 1983 Feb 01.
Article
em En
| MEDLINE
| ID: mdl-6342205
ABSTRACT
A heparin activated amidolytic enzyme capable of cleaving synthetic thrombin sensitive chromogenic substrates was identified from the plasma of the carp (Cyprinus carpio). Activation was inhibited by KCl, protamine sulphate and human plasma. Heparin was not required for the continued action of the enzyme. Active enzyme was irreversibly inhibited by DFP. The pH and temperature optima was studied and the enzyme semi purified by Sephadex G100 superfine and Sephadex A50 chromatography. An approximate MW of 62,000 was found. Activity generated by as little as 0.002 units of heparin per ml carp plasma was detected. The trivial name carpamidase is proposed for the enzyme.
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Base de dados:
MEDLINE
Assunto principal:
Peptídeo Hidrolases
/
Carpas
/
Cyprinidae
/
Heparina
/
Ativação Enzimática
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Thromb Res
Ano de publicação:
1983
Tipo de documento:
Article