Studies of DNA organization in hydrated spermidine-condensed DNA toruses and spermidine-DNA fibres.
J Biomol Struct Dyn
; 1(5): 1109-32, 1984 Mar.
Article
em En
| MEDLINE
| ID: mdl-6400814
ABSTRACT
We have investigated in vitro spermidine-condensed DNA preparations by both biochemical and freeze-etch electron microscopic approaches. These studies lead us to the conclusion that the reversibly condensed preparations, qualitatively described by Manning's counterion condensation theory, contain disk-like torus structures largely comprised of unidirectional, circumferentially wrapped DNA. Stereoscopic measurements on stereomicrographs of DNA torus and fibre objects have demonstrated the feasibility of directly measuring DNA writhe or, for that matter, mapping any secondary, tertiary or quaternary structure features of a hydrated macromolecular array in which the features can be differentially highlighted by low replica metal shadow levels.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Espermidina
Limite:
Animals
Idioma:
En
Revista:
J Biomol Struct Dyn
Ano de publicação:
1984
Tipo de documento:
Article