Replication forms of the gene-sized DNA molecules of hypotrichous ciliates.
Mol Cell Biol
; 3(9): 1562-6, 1983 Sep.
Article
em En
| MEDLINE
| ID: mdl-6415403
ABSTRACT
Using a method for obtaining DNA from 10 to 40 macronuclei for electron microscopy, we analyzed the structure of gene-sized, linear DNA molecules from S-phase macronuclei of two hypotrichous ciliates, Euplotes eurystomus and Styx sp. Three types of putative replicating intermediates were observed (i) molecules with a bubble close to one end, (ii) molecules with single forks, and (iii) molecules with two forks. We conclude that (i) each macronuclear DNA molecule replicates as an independent unit, (ii) the molecules contain an origin of replication close to one or both ends, and (iii) the mode of replication is bidirectional.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Cilióforos
/
Replicação do DNA
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Ano de publicação:
1983
Tipo de documento:
Article