Typing of subclasses and light chains of human monoclonal immunoglobulins by particle counting immunoassay (PACIA).
J Immunol Methods
; 69(2): 229-41, 1984 Apr 27.
Article
em En
| MEDLINE
| ID: mdl-6425415
ABSTRACT
The subclasses of monoclonal IgGs and IgAs were identified by particle-counting immunoassay. The principle of the test is the inhibition of the agglutinating activity of either specific antisera or monoclonal antibodies (for IgA only) on latex particles coated with a monoclonal IgG or IgA of known subclass. The feasibility of assay of polyclonal Ig subclasses was demonstrated. However, the anti-IgG2 antiserum cross-reacted with an allotype (nG4m(b)) of IgG4. The possibility of typing monoclonal Igs for light chains by the same technique was also demonstrated. Results are obtained in 30 min, and the method requires only small amounts of purified immunoglobulins (Igs) and antisera or monoclonal antibodies.
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Base de dados:
MEDLINE
Assunto principal:
Imunoglobulina A
/
Imunoglobulina G
/
Cadeias Leves de Imunoglobulina
/
Anticorpos Monoclonais
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1984
Tipo de documento:
Article