Differences in the in vitro growth pattern of fresh and cryopreserved granulo-monopoietic precursors.
Cryobiology
; 21(5): 486-90, 1984 Oct.
Article
em En
| MEDLINE
| ID: mdl-6499495
ABSTRACT
A study of the in vitro growth model of human granulo-monopoietic precursors (CFU-GM) before and after cryopreservation using both leukocyte feeder layers and GCT conditioned medium as the source of colony stimulating activity (CSA) is reported. The number of colonies produced with fresh cells was linearly related to the amount of marrow seeded with both CSA sources, whereas after cryopreservation this was true with feeder layers, and with GCT only at relatively high cell concentrations. This might indicate the production of granulopoietic stimulators on the part of a second population that is at least partly resistant to freezing. It seems more likely, however, that these results depend mainly on a sublethal damage to CFU-GM induced by freezing, thus making the cells hyporesponsive to some forms of CSA, such as those contained in GCT conditioned medium.
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Base de dados:
MEDLINE
Assunto principal:
Preservação de Tecido
/
Células-Tronco Hematopoéticas
/
Monócitos
/
Granulócitos
Limite:
Humans
Idioma:
En
Revista:
Cryobiology
Ano de publicação:
1984
Tipo de documento:
Article