Identification of the major protein adduct formed in rat liver after thioacetamide administration.
Cancer Res
; 41(9 Pt 1): 3430-5, 1981 Sep.
Article
em En
| MEDLINE
| ID: mdl-6790164
ABSTRACT
The in vivo covalent binding of the hepatocarcinogen thioacetamide to rat liver protein has been examined. Following administration of 3H- or 14C-labeled thioacetamide, the modified amino acids present in the hepatic cytosolic proteins were isolated by enzymatic digestion and ion-exchange chromatography. Approximately 70% of the radioactivity covalently bound to cytosolic protein was recovered in a compound which upon acid hydrolysis yielded lysine and radiolabeled acetate. Additional studies indicated the structure of this adduct was N-epsilon-acetyllysine.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Tioacetamida
/
Proteínas
/
Fígado
/
Acetamidas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1981
Tipo de documento:
Article