Development of a rapid microELISA assay for screening hybridoma supernatants for murine monoclonal antibodies.
J Immunol Methods
; 54(3): 291-6, 1982 Nov 12.
Article
em En
| MEDLINE
| ID: mdl-6983543
ABSTRACT
A micro enzyme-linked immunosorbent assay (ELISA) utilizing a filtration method has been developed which allows the rapid, simple, and sensitive detection of monoclonal antibodies that recognize either soluble or cell surface antigens. This assay involves the immobilization of target cells (or soluble antigen) onto glass fiber filter discs followed by an incubation with the test hybridoma supernatant and subsequent analysis by ELISA. A specially designed 96-well filtration device is employed which serves both as an incubation chamber and as a filtration manifold. This microELISA requires small volumes of antiserum, few target cells, and can be completed in less than 2 h. This assay is well suited for the rapid screening of murine hybridoma supernatants and can be adapted to detect monoclonal antibodies from other species.
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Base de dados:
MEDLINE
Assunto principal:
Hibridomas
/
Anticorpos Monoclonais
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Immunol Methods
Ano de publicação:
1982
Tipo de documento:
Article