Partial purification and properties of the cytoplasmic factors modulating adenylate cyclase activity in rat lung plasma membranes.
Biochim Biophys Acta
; 677(1): 153-9, 1981 Sep 18.
Article
em En
| MEDLINE
| ID: mdl-7295788
ABSTRACT
The adult rat lung supernatant contains some factors which markedly enhance adenylate cyclase activity in membranes (Nijjar, M.S. (1979) Biochim. Biophys. Acta 584, 43-50). These factors were separated into two less active components (peaks 1 and 2) by DEAE-cellulose chromatography. However, their recombination restored the full activation of adenylate cyclase. Further purification and characterization of these factors revealed that the activator in peak 1 contained two proteins of low (14 500) and high (65 000) molecular weight whereas the activator in peak 2 contained only one protein of 65 000. The kinetics of adenylate cyclase activation revealed that both the Km and V values were affected. The data also demonstrate that calmodulin was not involved in the cytoplasmic activation of adenylate cyclase in rat lungs.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Adenilil Ciclases
/
Citoplasma
/
Pulmão
Limite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Ano de publicação:
1981
Tipo de documento:
Article