[Site-specific scission of DNA of polytene chromosomes of Chironomus thummi in situ by the oligoadenylate alkylating derivatives]. / Induktsiia napravlennykh razryvov v DNK politennykh khromosom Chironomus thummi in situ pri deistvii alkiliruiushchikh proizvodnykh oligoadenilatov.

ABSTRACT

The possibility of site-specific scission of DNA in polytene chromosomes in situ by means of the method of complementarily addressed fragmentation is demonstrated. The fragmentation of polytene chromosomes of Chironomus thummi resulting from the alkylation of the denatured chromosomes with oligoadenylate derivatives, followed by scission of the DNA in specific sites and enlargement of nicks with exonucleases was investigated. Single-stranded regions were registered by means of luminescence microscopy after staining the chromosomes with acridine orange. The pattern of degradation of the chromosomes depends on the length of the oligoadenylate part of the reagents and is different from that obtained with uridine-5'-methylphosphate alkylating derivative which does not form any complexes with DNA. Oligoadenylates inhibit the action of the alkylating derivatives on the chromosomes.