Expression of an heterologous gene activating actinorhodin biosynthesis in Streptomyces lividans and Streptomyces coelicolor.
FEMS Microbiol Lett
; 116(3): 301-6, 1994 Mar 01.
Article
em En
| MEDLINE
| ID: mdl-7514144
ABSTRACT
Production of the blue-pigmented antibiotic actinorhodin resulted in activation in the non-producer strain Streptomyces lividans, but not in the natural producer strain Streptomyces coelicolor, when transformed with an heterologous activator gene from Streptomyces fradiae. The gene encodes a 132 nucleotide-long transcript, responsible for the actinorhodin production phenotype, and thought to act as a putative antisense RNA, which has been detected in the transformed S. lividans cultures by reverse transcription followed by cyclic amplification.
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Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Genes Bacterianos
/
Antibacterianos
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Espanha