Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma.
Cancer Epidemiol Biomarkers Prev
; 4(5): 509-13, 1995.
Article
em En
| MEDLINE
| ID: mdl-7549807
ABSTRACT
Prospective studies based on the storage of biological samples at low temperature have opened new perspectives in etiological research on cancer. In planning these studies a crucial question is to evaluate whether the long-term preservation of samples is able to affect the categorization of the subjects involved. In the frame of the ORDET project, a prospective study of hormones and diet in the etiology of breast cancer provided with a -80 degrees C biological bank, we have evaluated the stability of estradiol, free and total testosterone, and prolactin in serum and plasma samples over 3 years of cryoconservation. Study results showed that the subjects maintained almost the same rank by hormonal concentration throughout the 3-year period for all hormones. Looking at the stability over time, estradiol, prolactin, and total testosterone had fairly good performance for both serum and plasma. Serum-free testosterone increased in time up to 30%, whereas progesterone decreased by about 40% of the initial concentration. However, the reliability of the individual categorization by hormonal level suggests the validity of low temperature storage for epidemiological purposes, at least for hormonal parameters.
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Base de dados:
MEDLINE
Assunto principal:
Plasma
/
Progesterona
/
Prolactina
/
Testosterona
/
Preservação de Sangue
/
Criopreservação
/
Estradiol
Tipo de estudo:
Etiology_studies
/
Observational_studies
Limite:
Female
/
Humans
Idioma:
En
Revista:
Cancer Epidemiol Biomarkers Prev
Assunto da revista:
BIOQUIMICA
/
EPIDEMIOLOGIA
/
NEOPLASIAS
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Itália