Serial analysis of gene expression.
Science
; 270(5235): 484-7, 1995 Oct 20.
Article
em En
| MEDLINE
| ID: mdl-7570003
ABSTRACT
The characteristics of an organism are determined by the genes expressed within it. A method was developed, called serial analysis of gene expression (SAGE), that allows the quantitative and simultaneous analysis of a large number of transcripts. To demonstrate this strategy, short diagnostic sequence tags were isolated from pancreas, concatenated, and cloned. Manual sequencing of 1000 tags revealed a gene expression pattern characteristic of pancreatic function. New pancreatic transcripts corresponding to novel tags were identified. SAGE should provide a broadly applicable means for the quantitative cataloging and comparison of expressed genes in a variety of normal, developmental, and disease states.
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Base de dados:
MEDLINE
Assunto principal:
Pâncreas
/
RNA Mensageiro
/
Expressão Gênica
/
Técnicas Genéticas
Tipo de estudo:
Guideline
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Science
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos