Thermal stability of the polyheme cytochrome c3 superfamily.
FEBS Lett
; 373(3): 280-4, 1995 Oct 16.
Article
em En
| MEDLINE
| ID: mdl-7589483
ABSTRACT
The cytochrome c3 superfamily includes Desulfovibrio polyheme cytochromes c. We report the characteristic thermal stability parameters of the Desulfovibrio desulfuricans Norway (D.d.N.) cytochromes c3 (M(r) 13,000 and M(r) 26,000) and the Desulfovibrio vulgaris Hildenborough (D.v.H.) cytochrome c3 (M(r) 13,000) and high molecular mass cytochrome c (Hmc), as obtained with the help of electronic spectroscopy, voltammetric techniques and differential scanning calorimetry. The polyheme cytochromes are denatured over a wide range of temperatures the D.v.H. cytochrome c3 is highly thermostable (Td = 121 degrees C) contrary to the D.d.N. protein (Td = 73 degrees C). The thermostability of the polyheme cytochromes is redox state dependent. The results are discussed in the light of the structural and functional relationships within the cytochrome c3 superfamily.
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Base de dados:
MEDLINE
Assunto principal:
Grupo dos Citocromos c
/
Desulfovibrio
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
França