Effects of cryopreservation on subsets of fetal liver cells.
Bone Marrow Transplant
; 11(5): 395-8, 1993 May.
Article
em En
| MEDLINE
| ID: mdl-7684940
ABSTRACT
Human fetal livers from 6 to 13 weeks postconception were analysed before and after cryopreservation. The percentages of cell subsets, detected by MoAbs, did not change significantly after cryopreservation. Compared with BM, fetal liver contained significantly smaller subsets of cells identified by MoAbs, with two exceptions. Fetal liver contained a mean of 47% M5 positive cells versus 31% in BM, and there was no difference in the numbers of CD34+ cells. The colony-forming capacity was studied 53 colonies grew from 10(5) cells from fresh fetal liver compared with 51 colonies from cryopreserved cells. For fresh BM the corresponding value was 88 per 10(5) cells. Incubation time for fetal stem cells was 17-18 days while the corresponding time for BM cells was 8-10 days.
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Base de dados:
MEDLINE
Assunto principal:
Células da Medula Óssea
/
Células-Tronco Hematopoéticas
/
Antígenos CD
/
Criopreservação
/
Subpopulações de Linfócitos T
/
Fígado
Limite:
Humans
Idioma:
En
Revista:
Bone Marrow Transplant
Assunto da revista:
TRANSPLANTE
Ano de publicação:
1993
Tipo de documento:
Article
País de afiliação:
Suécia