Purification and characterization of the protein kinase eEF-2 isolated from rat liver cells.
Acta Biochim Pol
; 41(4): 421-7, 1994.
Article
em En
| MEDLINE
| ID: mdl-7732759
ABSTRACT
The elongation factor 2 (eEF-2) protein kinase was isolated from rat liver cells, purified and partly characterized. It was found that the enzyme exists in an inactive form in the homogenate of rat liver. The active fraction of kinase eEF-2 was obtained after removal of the inhibitory substance by hydroxyapatite column chromatography. The purified enzyme is an electrophoretically homogeneous protein with relative molecular mass of approximately 90,000 and isoelectric point, pI = 5.9. The enzyme specifically phosphorylates the elongation factor eEF-2 in the presence of calmodulin and Ca2+.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Quinases Dependentes de Cálcio-Calmodulina
/
Fígado
Limite:
Animals
Idioma:
En
Revista:
Acta Biochim Pol
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Polônia