Cloning and functional characterization of the human vasoactive intestinal peptide (VIP)-2 receptor.
Biochem Biophys Res Commun
; 209(2): 385-92, 1995 Apr 17.
Article
em En
| MEDLINE
| ID: mdl-7733904
ABSTRACT
A full-length clone encoding the human VIP-2 receptor was isolated from a human placenta cDNA library. The 1317-bp cDNA insert encodes a protein of 438 amino acids which is a member of the seven transmembrane domain G-protein-coupled receptor superfamily. Expression of the human VIP-2 receptor in COS-7 cells confered high affinity binding to [125I] VIP (IC50 = 0.93 nM), which was displaced by unlabeled PACAP-38 (IC50 = 6.2 nM). VIP and PACAP-38 were equipotent in stimulating accumulation of cAMP in COS-7 cells transfected with the human VIP-2 receptor. Northern blot analysis revealed two VIP-2 receptor mRNAs of 4.6 kb and 2.3 kb in size which were expressed predominantly in the human skeletal muscle and to a lesser extent in the human brain, heart, pancreas and placenta.
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Base de dados:
MEDLINE
Assunto principal:
Receptores de Peptídeo Intestinal Vasoativo
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos