Extensive amplification of bcr/abl fusion genes clustered on three marker chromosomes in human leukemic cell line K-562.
Leukemia
; 9(5): 858-62, 1995 May.
Article
em En
| MEDLINE
| ID: mdl-7769849
ABSTRACT
Using fluorescence in situ hybridization (FISH), we were able to demonstrate 22-24-fold amplification of the bcr/abl fusion gene in the human leukemic cell line K-562. About 60% of the amplified sequences are localized to a large acrocentric marker chromosome, with another 30% clustered on a small acrocentric chromosome. In addition to these two masked Ph chromosomes, the remaining bcr/abl fusion genes are located on a der(2) distal to band q33. G- and C-banding analysis revealed similar unique banding patterns in both masked Ph chromosomes and suggests that amplification occurred by tandem duplication of the bcr/abl fusion site. Because the number of bcr/abl fusion genes may be increasing over time, it is critical that researchers using K-562 cells should be aware of this extensive amplification.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 9
/
Cromossomos Humanos Par 22
/
Cromossomo Filadélfia
/
Amplificação de Genes
/
Genes abl
/
Proteínas de Fusão bcr-abl
/
Leucemia-Linfoma Linfoblástico de Células Precursoras
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Leukemia
Assunto da revista:
HEMATOLOGIA
/
NEOPLASIAS
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Estados Unidos