Quantitative determination of intracellular depolymerase activity in Pseudomonas oleovorans inclusions containing poly-3-hydroxyalkanoates with long alkyl substituents.
FEMS Microbiol Lett
; 118(3): 279-82, 1994 May 15.
Article
em En
| MEDLINE
| ID: mdl-7794414
ABSTRACT
Research regarding the accurate, quantitative degradation of novel poly-3-hydroxyalkanoates has been restricted by the absence of an appropriate monitoring technique. The calibration of a gas chromatograph to poly-3-hydroxyoctanoate reveals a linear relationship between the area under gas chromatograph tracings and polymer weight. With this new method, poly-3-hydroxy-octanoate granules isolated from Pseudomonas oleovorans, which were incubated at 30 degrees C in an alkaline buffer, exhibited a linear degradation rate. Degradation was inhibited by the presence of Triton X-100 and phenylmethylsulfonyl fluoride. The depolymerase was demonstrated to be associated with the polymer granule complex and most likely possessed serine residues at its active site.
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Base de dados:
MEDLINE
Assunto principal:
Poliésteres
/
Polímeros
/
Pseudomonas
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1994
Tipo de documento:
Article