A simple microassay for computing the hemolytic potency of drugs.
Biotechniques
; 17(6): 1148-53, 1994 Dec.
Article
em En
| MEDLINE
| ID: mdl-7873185
ABSTRACT
A simple microassay and computer program are described for determining the erythrocyte hemolytic potency of drugs in vitro. This microassay is sensitive for both micro as well as macro ranges of hemoglobin concentration. An ELISA reader has been adapted to read erythrocyte lysis (hemolysis), which reduces the number and culture of replicates. A computer program was developed that calculates parameters such as C50 (concentration of drug causing 50% hemolysis), C100 (concentration of drug causing 100% hemolysis) and beta (slope of the curve) and graphically expresses the hemolytic patterns of various drugs simultaneously. The program can obtain optical densities directly from a 96-well plate ELISA reader by interfacing the microplate reader to the computer or by using a keyboard. This method is useful for screening a large number of hemolytic drugs and requires lower amounts of test compounds. It may also be applicable to quantitative functional assays, such as complement-mediated hemolysis and enumeration of antibody-secreting cells. The program can be obtained from the authors on request.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Análise Numérica Assistida por Computador
/
Processamento Eletrônico de Dados
/
Eritrócitos
/
Hemólise
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Índia