Isolation, identification, and synthesis of Mas-MG-MT I, a novel peptide from the larval midgut of Manduca sexta (Lepidoptera: Sphingidae).
Arch Insect Biochem Physiol
; 28(2): 159-71, 1995.
Article
em En
| MEDLINE
| ID: mdl-7894053
ABSTRACT
A five-residue myotropic peptide, Manduca sexta midgut myotropin I (Mas-MG-MT I), was isolated from an extract of 800 midguts of fifth instar larvae of the tobacco hornworm, Manduca sexta. It was purified by reverse phase and normal phase HPLC. Myotropic activity was screened by a heterologous Locusta migratoria oviduct bioassay. Sequence analysis, amino acid composition analysis, and comparison of candidate synthetic peptides in the amide and acid form revealed the following primary structure Ala-Glu-Pro-Tyr-Thr-NH2. This is the first fully identified peptide isolated directly from the midgut of an insect species. Few significant sequence homologies with known vertebrate and invertebrate peptides have been found.
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Base de dados:
MEDLINE
Assunto principal:
Oligopeptídeos
/
Manduca
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Arch Insect Biochem Physiol
Assunto da revista:
BIOLOGIA
/
BIOQUIMICA
Ano de publicação:
1995
Tipo de documento:
Article
País de afiliação:
Bélgica