Loss of heterozygosity of chromosome 9p21 is associated with the immortal phenotype of neoplastic human head and neck keratinocytes.
Cancer Res
; 54(19): 5045-9, 1994 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-7923114
ABSTRACT
Human chromosomes 1,4,6, and 9 harbor genes which induce cellular senescence in vitro but a role for their inactivation in human tumors is not established. To investigate this we searched for loss of heterozygosity (LOH) on these chromosomes in keratinocyte cultures obtained from different stages of human squamous cell carcinoma progression. There was consistent LOH between markers D9S171 and D9S157 in 9 of 9 (100%) informative immortal cultures and in one line which entered crisis, but 0 of 7 informative senescent cultures showed LOH. These results suggest that inactivation of a gene at 9p21 is important but insufficient for human squamous cell carcinoma keratinocyte immortalization.
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Base de dados:
MEDLINE
Assunto principal:
Cromossomos Humanos Par 9
/
Carcinoma de Células Escamosas
/
Proteínas S100
/
Queratinócitos
/
Deleção Cromossômica
/
Neoplasias de Cabeça e Pescoço
Tipo de estudo:
Risk_factors_studies
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1994
Tipo de documento:
Article
País de afiliação:
Reino Unido